diff --git a/.gitignore b/.gitignore
index 2917cfbef9..9226eecb4c 100644
--- a/.gitignore
+++ b/.gitignore
@@ -1,7 +1,5 @@
 # Random seed file created by test scripts and sample programs
 seedfile
-# MBEDTLS_PSA_INJECT_ENTROPY seed file created by the test framework
-00000000ffffff52.psa_its
 # Log files created by all.sh to reduce the logs in case a component runs
 # successfully
 quiet-make.*
diff --git a/docs/proposed/config-split.md b/docs/proposed/config-split.md
index 1baab356b2..1ed3cc773f 100644
--- a/docs/proposed/config-split.md
+++ b/docs/proposed/config-split.md
@@ -247,7 +247,6 @@ PSA_WANT_\* macros as in current `crypto_config.h`.
 //#define MBEDTLS_PSA_CRYPTO_KEY_ID_ENCODES_OWNER
 //#define MBEDTLS_PSA_CRYPTO_SPM
 #define MBEDTLS_PSA_CRYPTO_STORAGE_C
-//#define MBEDTLS_PSA_INJECT_ENTROPY
 #define MBEDTLS_PSA_ITS_FILE_C
 #define MBEDTLS_PSA_KEY_STORE_DYNAMIC
 //#define MBEDTLS_PSA_STATIC_KEY_SLOTS
diff --git a/scripts/config.py b/scripts/config.py
index 417f6e25a2..3fc3614dc7 100755
--- a/scripts/config.py
+++ b/scripts/config.py
@@ -96,7 +96,6 @@ EXCLUDE_FROM_FULL = frozenset([
     'MBEDTLS_PSA_CRYPTO_EXTERNAL_RNG', # behavior change + build dependency
     'MBEDTLS_PSA_CRYPTO_KEY_ID_ENCODES_OWNER', # interface and behavior change
     'MBEDTLS_PSA_CRYPTO_SPM', # platform dependency (PSA SPM)
-    'MBEDTLS_PSA_INJECT_ENTROPY', # conflicts with platform entropy sources
     'MBEDTLS_RSA_NO_CRT', # influences the use of RSA in X.509 and TLS
     'MBEDTLS_SHA256_USE_A64_CRYPTO_ONLY', # interacts with *_USE_A64_CRYPTO_IF_PRESENT
     'MBEDTLS_SHA256_USE_ARMV8_A_CRYPTO_ONLY', # interacts with *_USE_ARMV8_A_CRYPTO_IF_PRESENT
diff --git a/tests/configs/user-config-for-test.h b/tests/configs/user-config-for-test.h
deleted file mode 100644
index f230fd3c5c..0000000000
--- a/tests/configs/user-config-for-test.h
+++ /dev/null
@@ -1,29 +0,0 @@
-/* TF_PSA_CRYPTO_USER_CONFIG_FILE for testing.
- * Only used for a few test configurations.
- *
- * Typical usage (note multiple levels of quoting):
- *     make CFLAGS="'-DTF_PSA_CRYPTO_USER_CONFIG_FILE=\"../tests/configs/user-config-for-test.h\"'"
- */
-
-/*
- *  Copyright The Mbed TLS Contributors
- *  SPDX-License-Identifier: Apache-2.0 OR GPL-2.0-or-later
- */
-
-#if defined(MBEDTLS_PSA_INJECT_ENTROPY)
-/* The #MBEDTLS_PSA_INJECT_ENTROPY feature requires two extra platform
- * functions, which must be configured as #MBEDTLS_PLATFORM_NV_SEED_READ_MACRO
- * and #MBEDTLS_PLATFORM_NV_SEED_WRITE_MACRO. The job of these functions
- * is to read and write from the entropy seed file, which is located
- * in the PSA ITS file whose uid is #PSA_CRYPTO_ITS_RANDOM_SEED_UID.
- * (These could have been provided as library functions, but for historical
- * reasons, they weren't, and so each integrator has to provide a copy
- * of these functions.)
- *
- * Provide implementations of these functions for testing. */
-#include <stddef.h>
-int mbedtls_test_inject_entropy_seed_read(unsigned char *buf, size_t len);
-int mbedtls_test_inject_entropy_seed_write(unsigned char *buf, size_t len);
-#define MBEDTLS_PLATFORM_NV_SEED_READ_MACRO mbedtls_test_inject_entropy_seed_read
-#define MBEDTLS_PLATFORM_NV_SEED_WRITE_MACRO mbedtls_test_inject_entropy_seed_write
-#endif /* MBEDTLS_PSA_INJECT_ENTROPY */
diff --git a/tests/psa-client-server/psasim/src/psa_sim_generate.pl b/tests/psa-client-server/psasim/src/psa_sim_generate.pl
index 5770deaa80..3eec226e16 100755
--- a/tests/psa-client-server/psasim/src/psa_sim_generate.pl
+++ b/tests/psa-client-server/psasim/src/psa_sim_generate.pl
@@ -27,7 +27,6 @@ my @skip_functions = (
     'mbedtls_psa_crypto_free', # redefined rather than wrapped
     'mbedtls_psa_external_get_random', # not in the default config, uses unsupported type
     'mbedtls_psa_get_stats', # uses unsupported type
-    'mbedtls_psa_inject_entropy', # not in the default config, generally not for client use anyway
     'mbedtls_psa_platform_get_builtin_key', # not in the default config, uses unsupported type
     'psa_get_key_slot_number', # not in the default config, uses unsupported type
     'psa_key_derivation_verify_bytes', # not implemented yet
diff --git a/tests/scripts/components-configuration-crypto.sh b/tests/scripts/components-configuration-crypto.sh
index 3d58895550..cb66e371cb 100644
--- a/tests/scripts/components-configuration-crypto.sh
+++ b/tests/scripts/components-configuration-crypto.sh
@@ -261,21 +261,6 @@ component_test_psa_external_rng_use_psa_crypto () {
     tests/ssl-opt.sh -f 'Default\|opaque'
 }
 
-component_test_psa_inject_entropy () {
-    msg "build: full + MBEDTLS_PSA_INJECT_ENTROPY"
-    scripts/config.py full
-    scripts/config.py set MBEDTLS_PSA_INJECT_ENTROPY
-    scripts/config.py set MBEDTLS_ENTROPY_NV_SEED
-    scripts/config.py set MBEDTLS_NO_DEFAULT_ENTROPY_SOURCES
-    scripts/config.py unset MBEDTLS_PLATFORM_NV_SEED_ALT
-    scripts/config.py unset MBEDTLS_PLATFORM_STD_NV_SEED_READ
-    scripts/config.py unset MBEDTLS_PLATFORM_STD_NV_SEED_WRITE
-    make CC=$ASAN_CC CFLAGS="$ASAN_CFLAGS '-DTF_PSA_CRYPTO_USER_CONFIG_FILE=\"../tests/configs/user-config-for-test.h\"'" LDFLAGS="$ASAN_CFLAGS"
-
-    msg "test: full + MBEDTLS_PSA_INJECT_ENTROPY"
-    make test
-}
-
 component_full_no_pkparse_pkwrite () {
     msg "build: full without pkparse and pkwrite"
 
diff --git a/tf-psa-crypto b/tf-psa-crypto
index 43ea7fa25c..893f536dae 160000
--- a/tf-psa-crypto
+++ b/tf-psa-crypto
@@ -1 +1 @@
-Subproject commit 43ea7fa25cd8a288c5b75dbb0b4eb47df6ffca8b
+Subproject commit 893f536dae31f358516de6d9e851da7c18f5f53e